Hot Air Oven Sterilization Protocols

Question

Temperature-time protocol for sterilization inside hot air oven:

Select an answer

Temperature-time protocol for sterilization inside hot air oven:

  1. $160^{\circ}C/1hour$
  2. $170^{0}C/40$ min
  3. $180^{\circ}C/20$ min
  4. All the above — Correct Answer
Explanation:
Correct Answer: All the above

All three temperature-time combinations are acceptable protocols for dry heat sterilisation using a hot air oven. Higher temperatures require shorter exposure times to achieve the same sterilising effect.

Hot Air Oven — Standard Protocols
Temperature Time Notes
160°C 1 hour Most common standard protocol
170°C 40 min Higher temp = shorter time
180°C 20 min Even higher temp = even shorter time
Comparison with Other Sterilisation Methods
Method Type Protocol
Autoclave Moist heat 121°C / 15 lbs / 15 min
Hot air oven Dry heat 160°C/1h OR 170°C/40min OR 180°C/20min
Muffle furnace Dry heat (ash) 600°C / 1–2 hours
Note on Feed Analysis
  • Hot air oven at 100°C for 1 hour is used for moisture determination of feed samples (proximate analysis) — different from sterilisation protocol
Why Other Options Are Wrong
  • Each individual protocol is correct on its own — all three are valid hot air oven sterilisation conditions, making "All the above" the complete answer

📚 About this Topic — Animal Nutrition

This multiple choice question is from Animal Nutrition, Animal Refresher. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Nutrition to strengthen your preparation.

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