Topics:
Animal Nutrition, Animal Refresher
Hot Air Oven Sterilization Protocols
Temperature-time protocol for sterilization inside hot air oven:
- $160^{\circ}C/1hour$
- $170^{0}C/40$ min
- $180^{\circ}C/20$ min
- All the above — Correct Answer
Explanation:
Correct Answer: All the above
All three temperature-time combinations are acceptable protocols for dry heat sterilisation using a hot air oven. Higher temperatures require shorter exposure times to achieve the same sterilising effect.
Hot Air Oven — Standard Protocols
| Temperature | Time | Notes |
|---|---|---|
| 160°C | 1 hour | Most common standard protocol |
| 170°C | 40 min | Higher temp = shorter time |
| 180°C | 20 min | Even higher temp = even shorter time |
Comparison with Other Sterilisation Methods
| Method | Type | Protocol |
|---|---|---|
| Autoclave | Moist heat | 121°C / 15 lbs / 15 min |
| Hot air oven | Dry heat | 160°C/1h OR 170°C/40min OR 180°C/20min |
| Muffle furnace | Dry heat (ash) | 600°C / 1–2 hours |
Note on Feed Analysis
- Hot air oven at 100°C for 1 hour is used for moisture determination of feed samples (proximate analysis) — different from sterilisation protocol
Why Other Options Are Wrong
- Each individual protocol is correct on its own — all three are valid hot air oven sterilisation conditions, making "All the above" the complete answer
📚 About this Topic — Animal Nutrition
This multiple choice question is from Animal Nutrition, Animal Refresher. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Nutrition to strengthen your preparation.