Topics:
Animal Refresher, Animal Reproduction
Match the Column – Artificial Vagina Models & Cryopreservation
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Question (English)
Match the column — Artificial vagina models and semen cryopreservation materials:
| TABLE-I | TABLE-II |
|---|---|
| 1. Danish model of artificial vagina | a. Glycerol |
| 2. Norwegian model of artificial vagina | b. Stallion |
| 3. Mississippi model of artificial vagina | c. Bull |
| 4. Most commonly used cryogen | d. Boar |
| 5. Most commonly used cryoprotective agent | e. Liquid N₂ |
- 1-c, 2-d, 3-b, 4-e, 5-a ✓ Correct
- 1-b, 2-c, 3-d, 4-e, 5-a
- 1-c, 2-b, 3-d, 4-e, 5-a
- 1-d, 2-c, 3-b, 4-e, 5-a
Explanation:
Correct Answer: 1-c, 2-d, 3-b, 4-e, 5-a
Detailed Explanation
- 1. Danish model AV → (c) Bull: The Danish model artificial vagina is the most widely used for bull semen collection in AI centers. It consists of a rigid outer tube with a rubber liner, water-filled cavity, and a collection tube.
- 2. Norwegian model AV → (d) Boar: The Norwegian model is designed for boar semen collection — it accommodates the large volume of boar ejaculate (200–250 ml).
- 3. Mississippi model AV → (b) Stallion: The Mississippi model (also called the Colorado model in some texts) is used for stallion semen collection — designed to handle the large semen volume (~125 ml).
- 4. Most commonly used cryogen → (e) Liquid N₂ (LN₂): Liquid nitrogen at −196°C is the standard cryogen used for long-term storage of frozen semen straws.
- 5. Most commonly used cryoprotective agent → (a) Glycerol: Glycerol (5–7% in bull semen extender) is the standard cryoprotectant — it penetrates cells and prevents ice crystal formation during freezing.