Specific Site of Semen Deposition in Cow (AI)

The site of semen deposition in a cow during artificial insemination is:

Correct Answer: Utero-cervix (mid-cervix)

In cows, the standard recommended site for semen deposition during AI is the utero-cervix — specifically at the internal os (cervico-uterine junction / mid-cervix). This is done using the rectovaginal technique where one hand is inserted rectally to guide the cervix onto the insemination pipette.

Rectovaginal Technique in Cows

• The operator's arm is inserted into the rectum to palpate and stabilize the cervix.
• The insemination pipette is guided through the vagina and advanced through the cervical canal to the internal os.
• Semen is deposited at the utero-cervical junction for optimal results.

Why Utero-cervix and Not Uterus?

• Deep uterine horn insemination is not routinely practiced in cattle.
• The utero-cervical junction provides the best balance — it's deep enough to be close to where sperm travel up to the uterus, yet avoids potential uterine damage.