Topics:
Animal Nutrition, Animal Refresher
Standard Autoclave Protocol
Temperature-time protocol for sterilization inside autoclave:
- $121^{0}C/15$ lbs/15 min — Correct Answer
- $121^{0}C/15~lbs/15$ sec
- $121^{0}C/21$ lbs/15 min
- 111°C/15 lbs/15 min
Explanation:
Correct Answer: 121°C / 15 lbs / 15 min
The standard temperature-time protocol for sterilization inside an autoclave (moist heat sterilization) is 121°C / 15 lbs (pressure) / 15 minutes. This is also the condition used for autoclaving soybean to destroy trypsin inhibitors.
Temperature-Time Protocols — All Instruments
| Instrument | Temperature | Pressure | Time |
|---|---|---|---|
| Autoclave | 121°C | 15 lbs/in² | 15 min |
| Hot air oven | 160°C | — | 1 hour |
| Hot air oven (alt) | 170°C | — | 40 min |
| Hot air oven (alt) | 180°C | — | 20 min |
| Muffle furnace | 600°C | — | 1–2 hours |
Practical Applications of Autoclave Conditions
- Sterilisation of culture media, surgical instruments, glassware
- Destruction of trypsin inhibitor in soybean (anti-nutritional factor)
- Destruction of Ricin in castor bean (by moist heat)
Why Other Options Are Wrong
- 121°C/15 lbs/15 sec → 15 seconds is far too short; sterilisation is not achieved
- 121°C/21 lbs/15 min → 21 lbs pressure is not the standard autoclave pressure
- 111°C/15 lbs/15 min → lower temperature; inadequate for complete sterilisation
📚 About this Topic — Animal Nutrition
This multiple choice question is from Animal Nutrition, Animal Refresher. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Nutrition to strengthen your preparation.
