Test to Detect PMSG in Serum of Pregnant Mare

Question

Which of the following tests is used to detect PMSG in the serum of a pregnant mare?

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Question (English)

Which of the following tests is used to detect PMSG in the serum of a pregnant mare?

  1. Casoni test
  2. Cuboni test
  3. Aschheim-Zondek (A-Z) test ✓ Correct
  4. All of the above
Explanation:
Correct Answer: Aschheim-Zondek (A-Z) test

The Aschheim-Zondek (A-Z) test is a biological test used to detect PMSG (eCG) in the blood/serum of a pregnant mare. In the test, the mare's serum is injected into immature female mice — if PMSG is present, it causes follicular development and ovulation in the test mice (indicating pregnancy in the mare).

Summary: Pregnancy Tests in Mare
  • Cuboni test: Detects estrogen in urine (from ~150 days gestation)
  • A-Z test: Detects PMSG in serum (from ~40–120 days gestation)
Diagnostic Window
  • PMSG is detectable in mare serum from day 40 to day 120–150 of pregnancy, so the A-Z test is useful during this period.
  • Beyond 150 days, estrogen-based tests (Cuboni) are more reliable.

📚 About this Topic — Animal Refresher

This multiple choice question is from Animal Refresher, Animal Reproduction. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Refresher to strengthen your preparation.

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