Topics:
Animal Refresher, Animal Reproduction
Thawing Temperature for Frozen Semen
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Question (English)
Thawing temperature for frozen semen is:
- 37–40°C for 30 seconds ✓ Correct
- 37–40°C for 60 seconds
- 37–40°C for 15 seconds
- 50°C for 60 seconds
Explanation:
Correct Answer: 37–40°C for 30 seconds
Standard protocol for thawing frozen bull semen is to immerse the straw in a water bath at 37–40°C for 30 seconds. This rapid thaw minimizes re-crystallization injury to spermatozoa.
Why Rapid Thawing is Important
- Slow thawing at room temperature allows ice crystals to reform inside sperm cells (recrystallization), causing cell membrane rupture and death.
- Rapid thawing at 37–40°C quickly melts ice crystals before they can cause significant damage.
Post-Thaw Quality Parameters (Bull Semen)
- Post-thaw motility should be ≥ 40%
- Normal morphology ≥ 80%
- Insemination should be done within 15 minutes of thawing for best conception rates.
📚 About this Topic — Animal Refresher
This multiple choice question is from Animal Refresher, Animal Reproduction. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Refresher to strengthen your preparation.
