Test to Detect Estrogen in Urine of Pregnant Mare

Question

In case of pregnancy diagnosis in the mare, which test is used to detect estrogen in urine?

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Question (English)

In case of pregnancy diagnosis in the mare, which test is used to detect estrogen in urine?

  1. Casoni test
  2. Cuboni test ✓ Correct
  3. Aschheim-Zondek (A-Z) test
  4. All of the above
Explanation:
Correct Answer: Cuboni test

The Cuboni test is used to detect estrogen (specifically estrogens/conjugated estrogens) in the urine of a pregnant mare. It is a colorimetric test — the urine is treated with sulfuric acid and resorcinol; a positive result shows a green fluorescence under UV light.

Pregnancy Diagnosis Tests in Mare
  • Cuboni test: Detects estrogen in urine → positive from ~150 days of pregnancy
  • Aschheim-Zondek (A-Z) test: Detects PMSG in serum → positive from ~40 days to ~120 days of pregnancy
  • Rectal palpation and ultrasonography: Most reliable and earliest physical methods
Casoni Test

The Casoni test is actually an intradermal skin test used for the diagnosis of Echinococcosis (hydatid disease) — it has nothing to do with pregnancy diagnosis.

📚 About this Topic — Animal Refresher

This multiple choice question is from Animal Refresher, Animal Reproduction. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Refresher to strengthen your preparation.

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