Proximate Principle Calculated by Difference

Question

Which of the following proximate principle is not determined by analysis but simply calculated by difference?

Select an answer

Which of the following proximate principle is not determined by analysis but simply calculated by difference?

  1. CF
  2. NFE — Correct Answer
  3. CP
  4. Total ash
Explanation:
Correct Answer: NFE

NFE (Nitrogen-Free Extract) is the only proximate principle that is not directly determined by chemical analysis — it is simply calculated by subtraction from the other measured proximate principles.

NFE Calculation
  • NFE% (DM basis) = 100 – (CP% + EE% + CF% + Ash%)
  • All other proximate principles are subtracted from 100 to give NFE
  • NFE represents easily digestible carbohydrates (monosaccharides, disaccharides)
Proximate Principles — Methods of Determination
Proximate Principle Method
Crude Protein (CP) Kjeldahl method (direct analysis)
Ether Extract (EE) Soxhlet apparatus (direct analysis)
Crude Fibre (CF) Sequential acid-alkali digestion (direct analysis)
Total Ash Muffle furnace at 600°C/2 hrs (direct analysis)
Moisture/Water Hot air oven at 100°C/1 hr (direct analysis)
NFE Calculated by difference — NOT directly analysed
Why Other Options Are Wrong
  • CF → determined by direct analysis (acid-alkali digestion method)
  • CP → determined by Kjeldahl method directly
  • Total ash → determined by muffle furnace directly

📚 About this Topic — Animal Nutrition

This multiple choice question is from Animal Nutrition, Animal Refresher. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Nutrition to strengthen your preparation.

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