Components Excluded from ADF

Question

In detergent method of forage analysis, Acid detergent fiber (ADF) does NOT include:

Select an answer

In detergent method of forage analysis, Acid detergent fiber (ADF) does NOT include:

  1. Cellulose
  2. Hemicelluloses — Correct Answer
  3. Lignin
  4. Silica
Explanation:
Correct Answer: Hemicellulose

In the Van Soest detergent method, Acid Detergent Fibre (ADF) contains cellulose, lignin, and silica — but NOT hemicellulose. Hemicellulose is the component that distinguishes NDF from ADF (ADS = NDF − ADF = Hemicellulose).

Van Soest Method — Fibre Fractions
Fraction Components
NDF (Neutral Detergent Fibre) Hemicellulose + Cellulose + Lignin + Silica (entire plant cell wall)
ADF (Acid Detergent Fibre) Cellulose + Lignin + Silica (NOT hemicellulose)
ADS (Acid Detergent Soluble) Hemicellulose (= NDF − ADF)
NDS (Neutral Detergent Solubles) Soluble carbohydrates, starch, proteins, pectins (cell contents)
Key Relationship
  • NDF − ADF = Hemicellulose (the ADF-soluble = ADS fraction)
  • ADF is boiled with Acid Detergent Solution: CTAB in 1N H₂SO₄
  • NDF is boiled with Neutral Detergent (Sodium Lauryl Sulphate) at pH 7
Why Other Options Are Wrong
  • Cellulose → IS included in ADF
  • Lignin → IS included in ADF (most indigestible component)
  • Silica → IS included in ADF (Acid Insoluble Ash portion)

📚 About this Topic — Animal Nutrition

This multiple choice question is from Animal Nutrition, Animal Refresher. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Nutrition to strengthen your preparation.

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