Calibration Standard for Bomb Calorimetry
Reference standard used in Bomb calorimeter is:
- Butyric acid
- Benzoic acid — Correct Answer
- Cyclophosphamide
- Carotene
Correct Answer: Benzoic acid
A Bomb Calorimeter measures the Gross Energy (GE) of feed samples by complete combustion. It must be calibrated using a substance with a precisely known heat of combustion. Benzoic acid (26.43 kJ/g or 6.318 kcal/g) is the universally accepted reference standard because it is pure, stable, completely combustible, and has a highly reliable GE value.
Energy Partitioning — Key Values from Notes
| Energy Value | Conversion |
|---|---|
| 1 kg TDN | 4.4 Mcal/g Digestible Energy (DE) |
| 1 kg TDN | 3.6 Mcal/g Metabolic Energy (ME) [DE × 0.82 = ME] |
| 1 kg TDN | 0.86 Starch Equivalent |
| Faecal energy loss (roughage, ruminant) | 40–50% of GE intake |
| CH₄ energy loss | 7% of GE intake |
| CH₄ energy content | 13.34 kcal/g |
Physiological Fuel Values (PFV by Atwater)
| Nutrient | GE (kcal/g) | PFV (kcal/g) |
|---|---|---|
| Carbohydrate | 4.15 | 4.0 |
| Fat | 9.4 | 9.0 |
| Protein | 5.65 | 4.0 |
📚 About this Topic — Animal Nutrition
This multiple choice question is from Animal Nutrition, Animal Refresher. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Nutrition to strengthen your preparation.