Darkening of Egg Yolk in Hard-Boiled Egg

Question

Darkening of egg yolk in a hard-boiled egg is due to the formation of:

Select an answer

Darkening of egg yolk in a hard-boiled egg is due to the formation of:

  1. H₂S
  2. Iron sulfide — Correct Answer
  3. H₂ gas
  4. Riboflavin
Explanation:
Correct Answer: Iron sulfide (FeS)

The dark greenish-grey ring around the yolk of a hard-boiled egg is caused by Iron Sulfide (FeS), which forms when hydrogen sulfide (H₂S) — released from the albumin during overcooking — reacts with iron (Fe) present in the yolk.

Chemical Reaction

H₂S (from albumin) + Fe (from yolk) → FeS (Iron Sulfide) — dark compound

Prevention
  • Do not overcook eggs.
  • Place eggs in cold water immediately after boiling — this stops further H₂S diffusion into the yolk.

The H₂S is generated by the breakdown of sulfur-containing amino acids (cysteine, methionine) in albumin upon heating.

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This multiple choice question is from Animal Refresher, Poultry Science. It has 4 options with a detailed explanation of the correct answer. Practice more MCQs from Animal Refresher to strengthen your preparation.

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